HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity

Quimica nova

Santos, Lourdes C. dos¹  Amorim, Marcelo R. de¹  Rinaldo, Daniel²  Amaral, Fabiano P. do³  Vilegas, Wagner³  Vieira Jr, Gerardo M.⁴  Magenta, Mara A. G.⁵ 

[1] Universidade Estadual Paulista, Araraquara, Brasil;Universidade Estadual Paulista, Bauru, Brasil;Universidade Estadual Paulista, São Vicente, Brasil;Universidade Federal de Mato Grosso, Sinop, Brasil;Universidade Santa Cecília, Santos, Brasil

关键词: Eriocaulaceae;    Tonina fluviatilis;    flavonoids;    HPLC-DAD;    antiradical activity.;   

DOI  :  10.5935/0100-4042.20140193

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid).

【 授权许可】

CC BY