FORENSIC CASES IN THE NORTH OF CHILE: DETERMINATION OF ANTIDEPRESSANT DRUGS IN HUMAN WHOLE BLOOD

Journal of The Chilean Chemical Society

ZAMBRA, CARMEN¹  BUC CALDERON, PEDRO¹  BENITES, JULIO²  BRAVO, FELIPE²  VENEGAS, KARINA²  RIOS, DAVID² 

[1] Servicio Médico Legal, Iquique, Chile;Universidad Arturo Prat, Iquique, Chile

关键词: antidepressants;    gas chromatography;    mass selective detector;    ì;    -;    electron capture detector;    solid-;    phase extraction. ;    INTRODUCTION;   

DOI  :  10.4067/S0717-97072013000200020

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

The consumption of antidepressant drugs has increased in these last years, leading to severe and lethal poisonings. In this work, analytical tools, namely GC/MSD and GC/NPD µECD, were used to identify and quantify several antidepressant drugs including amitriptyline, imipramine, sertraline, fluoxetine, and citalopram in forensic cases in the North of Chile during 2008-2011. Drugs were analyzed in biological arrays like blood. A solid phase extraction by Bond Elut Certify columns was applied in all these processes. Fluoxetine and sertraline were derivatized with pentafluoropropionic anhydride. Prazepam was used as internal standard (IS). The limit of detection (LOD) in blood were 0.5 - 20.07 ng/mL. The average extraction rate was 89.39% in blood. The relative standard deviation (RSD) was less than 3.6%, while the intra-day accuracy was < 5.5% and the inter-day was < 2.4%, referred to RSD. The procedures we have developed allow the quantification of drugs even at low therapeutic doses, a very important issue taking into account the nature of the analyzed arrays.

【 授权许可】

Unknown