VALIDATION OF A METHOD TO DETECT COCAINE AND BENZOYLECGONINE IN HUMAN WHOLE BLOOD BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND APPLICATION IN BODY PACKERS AND STUFFERS CASES IN THE NORTH OF CHILE

Journal of The Chilean Chemical Society

CONTZEN, MARÍA CECILIA¹  BUC CALDERON, PEDRO¹  MOLLO, JULIO²  BENITES, JULIO²  BRAVO, FELIPE² 

[1] Servicio Médico Legal, Iquique, Chile;Universidad Arturo Prat, Iquique, Chile

关键词: Benzoylecgonine;    cocaine;    gas chromatography;    mass selective detector;    solid-;    phase extraction;    body packers-;    stuffers.;   

DOI  :  10.4067/S0717-97072012000300012

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

The present paper describes a method for the simultaneous determination of cocaine and benzoylecgonine in human whole blood. The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction and quantification by GC/MS with electron impact ionization using helium as carrier gas. Quantification was performed using cocaine-d3 as internal standard in selected ion monitoring mode. The method is very simple, rapid and sensitive. The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection were 3.6 ng/mL for cocaine and 6.8 ng/mL for benzoylecgonine. The method was applied to blood samples removed at autopsy from body packers and stuffers cases in the I - IV and XV regions of Chile during 2008-2010.

【 授权许可】

Unknown