PLoS Pathogens | |
Tubule-Guided Cell-to-Cell Movement of a Plant Virus Requires Class XI Myosin Motors | |
Valerian V. Dolja1  Alexander Lerich2  Corinne Schmitt-Keichinger2  Christophe Ritzenthaler2  Khalid Amari2  | |
[1] Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, United States of America;Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg, France | |
关键词: Myosins; Motor proteins; Membrane proteins; Leaves; Immunoblot analysis; Virions; Plant viral pathogens; Tobacco mosaic virus; | |
DOI : 10.1371/journal.ppat.1002327 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral movement proteins (MP) modify PD to allow passage of the virus particles or nucleoproteins. This passage occurs via several distinct mechanisms one of which is MP-dependent formation of the tubules that traverse PD and provide a conduit for virion translocation. The MP of tubule-forming viruses including Grapevine fanleaf virus (GFLV) recruit the plant PD receptors called Plasmodesmata Located Proteins (PDLP) to mediate tubule assembly and virus movement. Here we show that PDLP1 is transported to PD through a specific route within the secretory pathway in a myosin-dependent manner. This transport relies primarily on the class XI myosins XI-K and XI-2. Inactivation of these myosins using dominant negative inhibition results in mislocalization of PDLP and MP and suppression of GFLV movement. We also found that the proper targeting of specific markers of the Golgi apparatus, the plasma membrane, PD, lipid raft subdomains within the plasma membrane, and the tonoplast was not affected by myosin XI-K inhibition. However, the normal tonoplast dynamics required myosin XI-K activity. These results reveal a new pathway of the myosin-dependent protein trafficking to PD that is hijacked by GFLV to promote tubule-guided transport of this virus between plant cells.
【 授权许可】
CC BY
【 预 览 】
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RO201902015391917ZK.pdf | 1322KB | download |