期刊论文详细信息
Scientific Research and Essays
Assessment of groundwater quality in the rural areas of the North West Province, South Africa
Mpenyana-Monyatsi L.1 
关键词:  ;    Groundwater;    physical;    chemical;    microbiological water quality.;   
DOI  :  10.5897/SRE11.906
学科分类:社会科学、人文和艺术(综合)
来源: Academic Journals
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【 摘 要 】

The aim of this study is to assess the quality of the groundwater currently supplied to the rural communities of the North West Province. Groundwater samples collected from 100 boreholes in these rural areas were subjected to culture-based methods using selective media for the isolation of faecal and total coliform bacteria. Molecular analysis targeting 16S rRNA gene, and the restriction fragment-length polymorphism (RFLP) and sequence analysis of amplified gene for differentiating between species and strains of bacteria, were applied to selected coliform isolates. The physico-chemical parameters such as pH, temperature, total dissolved solids (TDS), turbidity and concentrations of chloride, sulphates, nitrate, fluoride, magnesium, sodium and calcium were also determined. The results revealed that 83% of the boreholes did not comply with the fluoride limit of SANS 241 (0 to 1 mg/l), 40% did not comply with the nitrate limit (0 to 6 mg/l as N), 45% did not comply with the magnesium limit (0 to 30 mg/l as Mg), 43% did not comply with the calcium limit (0 to 32 mg/l as Ca), 31% did not comply with TDS and 47% did not comply with the turbidity limit (<1 NTU). The results also indicated that 23 and 86% of the boreholes did not comply with the limits set by the national guidelines (SANS 241 and DWAF) in terms of faecal (0 cfu/100 ml) and total coliforms (0 to 5 cfu/100 ml), respectively. The results of the molecular study revealed that out of 100 boreholes, 51% tested positive forCitrobacter freundii, 28% forSerratia marcescens, 12% forMorganella morganii, 8% forSalmonella enterica, 7% forAeromonas veronii, 5% for Bacillus cereus, 2% forEnterobacter cloacaeand 1% forEscherichia coliandShigella dysenteriae. The findings of this study showed convincing evidence that some groundwater supplies in rural areas of North West pose a serious health risk to consumers.

【 授权许可】

CC BY   

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