Novel Positive-Charged Nanoparticles for Efficient Magnetic Resonance Imaging of Islet Transplantation

Cell Medicine

Koichi Oishi¹  Yoshitaka Miyamoto¹  Hiroshi Yukawa¹  Shuji Hayashi¹  Hirofumi Noguchi²  Makoto Sawada³  Kenji Ono³  Katsutoshi Murase⁴  Hiroaki Saito⁴ 

[1] * Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya, Japan;* Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya, Japan† Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;§ Department of Brain Functions, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan;‡ Nagoya Research Laboratory, MEITO Sangyo Co., Ltd., Nagoya, Japan

关键词: Cationic nanoparticles;    Islet transplantation;    Magnetic resonance imaging (MRI);    In vivo imaging;    Dextran;   

DOI  :  10.3727/215517912X639397

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Significant graft loss immediately after islet transplantation occurs due to immunological and nonimmunological events. Magnetic resonance imaging (MRI) is an attractive potential tool for monitoring islet mass in vivo. Although an efficient uptake of MRI contrast agent is required for islet cell labeling, commercially available magnetic nanoparticles are not efficiently transduced into cells. In this study, we developed six kinds of novel magnetic iron oxide nanoparticles, which are electrically charged by cationic end-group substitution of dextran. Each of the nanoparticles consisted of a small monocrystalline, superparamagnetic iron oxide core that is stabilized by a cross-linked aminated dextran coating to improve stability. We also used three different commercially available nanoparticles for controls. The labeling efficiency of the novel nanoparticles was evaluated, and the feasibility of the imaging by MRI was assessed. The positive-charged nanoparticles were transduced into a β-cell line, MIN6 cells, but not three commercially available nanoparticles. MRI showed a marked decrease in signal intensity on T1- and T2-weighted images at the site of the labeled cells in vitro. These data suggest that novel positive-charged nanoparticles could be useful MRI contrast agents to monitor islet mass after transplantation.

【 授权许可】

CC BY