The Nuclear Protein Sge1 of Fusarium oxysporum Is Required for Parasitic Growth

PLoS Pathogens

Caroline B. Michielse¹  Martijn Rep¹  Erik M. M. Manders¹  Linda Reijnen¹  Sonja Boas¹  Ringo van Wijk¹  Claude Alabouvette²  Chantal Olivain² 

[1] Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;UMR 1229 INRA Université de Bourgogne Microbiologie du Sol et de l'Environnement, Dijon, France

关键词: Deletion mutagenesis;    Gene expression;    Plant fungal pathogens;    Pathogenesis;    Tomatoes;    Polymerase chain reaction;    Fusarium oxysporum;    Fungi;   

DOI  :  10.1371/journal.ppat.1000637

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Dimorphism or morphogenic conversion is exploited by several pathogenic fungi and is required for tissue invasion and/or survival in the host. We have identified a homolog of a master regulator of this morphological switch in the plant pathogenic fungus Fusarium oxysporum f. sp. lycopersici. This non-dimorphic fungus causes vascular wilt disease in tomato by penetrating the plant roots and colonizing the vascular tissue. Gene knock-out and complementation studies established that the gene for this putative regulator, SGE1 (SIX Gene Expression 1), is essential for pathogenicity. In addition, microscopic analysis using fluorescent proteins revealed that Sge1 is localized in the nucleus, is not required for root colonization and penetration, but is required for parasitic growth. Furthermore, Sge1 is required for expression of genes encoding effectors that are secreted during infection. We propose that Sge1 is required in F. oxysporum and other non-dimorphic (plant) pathogenic fungi for parasitic growth.

【 授权许可】

CC BY