Simultaneous determination of serotonin precursors - tryptophan and 5-hidroxytryptophan - in coffee

Quimica nova

Martins, Ana Carolina C. L.¹  Silva, Tarliane M.¹  Gloria, M. Beatriz A.¹ 

[1] Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

关键词: coffee;    serotonin;    tryptophan.;   

DOI  :  10.1590/S0100-40422010000200016

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Epidemiologicalstudies attributed positive effects in the central nervous system (CNS) to coffee.Among possible active constituents, serotonin, a neurotransmitter in the CNS,is present; but dietary sources do not cross the blood-brain barrier. Tryptophanand 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brainconcentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehydeand fluorimetric detection before and after hydrolysis with NaOH and extractionwith methanol:water was developed for the simultaneous determination of thesecompounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL),precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), andlinear from 0.3 to 40 μg/mLfor both compounds. It was applied to green and roasted arabica and robustacoffees.

【 授权许可】

CC BY