Dual Analysis of the Murine Cytomegalovirus and Host Cell Transcriptomes Reveal New Aspects of the Virus-Host Cell Interface

PLoS Pathogens

Vanda Juranic Lisnic¹  Stipan Jonjic¹  Marina Babic Cac¹  Tihana Trsan¹  Berislav Lisnic²  Joanne Trgovcich³  Chitrangada Das Mukhopadhyay³  Charles H. Cook³  Adam Mefferd³ 

[1] Department of Histology and Embryology and the Center for Proteomics, University of Rijeka School of Medicine, Rijeka, Croatia;Laboratory of Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia;The Department of Surgery, The Ohio State University, Columbus, Ohio, United States of America

关键词: Complementary DNA cloning;    Genomic libraries;    DNA libraries;    RNA sequencing;    Human cytomegalovirus;    Transcriptome analysis;    Non-coding RNA sequences;    Gene expression;   

DOI  :  10.1371/journal.ppat.1003611

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Major gaps in our knowledge of pathogen genes and how these gene products interact with host gene products to cause disease represent a major obstacle to progress in vaccine and antiviral drug development for the herpesviruses. To begin to bridge these gaps, we conducted a dual analysis of Murine Cytomegalovirus (MCMV) and host cell transcriptomes during lytic infection. We analyzed the MCMV transcriptome during lytic infection using both classical cDNA cloning and sequencing of viral transcripts and next generation sequencing of transcripts (RNA-Seq). We also investigated the host transcriptome using RNA-Seq combined with differential gene expression analysis, biological pathway analysis, and gene ontology analysis.

【 授权许可】

CC BY