Novel Polyomaviruses of Nonhuman Primates: Genetic and Serological Predictors for the Existence of Multiple Unknown Polyomaviruses within the Human Population

PLoS Pathogens

Jörg Hofmann¹  Nelly Scuda¹  Christophe Boesch²  Michael A. Jarvis²  Chantal Akoua-Koffi²  Sébastien Calvignac-Spencer³  Diana Wevers⁴  Kenneth N. Cameron⁵  Martha Robbins⁶  Siv Aina J. Leendertz⁷  Emmanuel Couacy-Hymann⁸  Fabian H. Leendertz⁸  Nadege Freda Madinda⁸  Bernhard Ehlers⁸  Ugo Moens⁹  Edgard Valerie Adjogoua^1,10  Lawrence Mugisha^1,11 

[1] Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany;Department of Primatology, Max Planck Institute, for Evolutionary Anthropology, Leipzig, Germany;EcoHealth Research Group, Conservation & Ecosystem Health Alliance (CEHA), Kampala, Uganda;Institut Pasteur Côte d'Ivoire, Abidjan, Côte d'Ivoire;Institute of Virology, Charité - Universitätsmedizin Berlin, Berlin, Germany;LANADA/Laboratoire Central de la Pathologie Animale, Bingerville, Côte d'Ivoire;Mountain Gorilla Veterinary Project, Inc., Maryland, Baltimore, Maryland, United States of America;Project 23 “Epidemiology of Highly Pathogenic Microorganisms,” Robert Koch Institute, Berlin, Germany;School of Biomedical & Biological Sciences, University of Plymouth, Plymouth, United Kingdom;University Teaching Hospital Bouaké, Bouaké, Côte d'Ivoire;University of Tromsø, Faculty of Health Sciences, Department of Medical Biology, Tromsø, Norway

关键词: Polyomaviruses;    Chimpanzees;    Phylogenetic analysis;    Primates;    Sequence motif analysis;    Gorillas;    Sequence alignment;    Serology;   

DOI  :  10.1371/journal.ppat.1003429

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Polyomaviruses are a family of small non-enveloped DNA viruses that encode oncogenes and have been associated, to greater or lesser extent, with human disease and cancer. Currently, twelve polyomaviruses are known to circulate within the human population. To further examine the diversity of human polyomaviruses, we have utilized a combinatorial approach comprised of initial degenerate primer-based PCR identification and phylogenetic analysis of nonhuman primate (NHP) polyomavirus species, followed by polyomavirus-specific serological analysis of human sera. Using this approach we identified twenty novel NHP polyomaviruses: nine in great apes (six in chimpanzees, two in gorillas and one in orangutan), five in Old World monkeys and six in New World monkeys. Phylogenetic analysis indicated that only four of the nine chimpanzee polyomaviruses (six novel and three previously identified) had known close human counterparts. To determine whether the remaining chimpanzee polyomaviruses had potential human counterparts, the major viral capsid proteins (VP1) of four chimpanzee polyomaviruses were expressed in E. coli for use as antigens in enzyme-linked immunoassay (ELISA). Human serum/plasma samples from both Côte d'Ivoire and Germany showed frequent seropositivity for the four viruses. Antibody pre-adsorption-based ELISA excluded the possibility that reactivities resulted from binding to known human polyomaviruses. Together, these results support the existence of additional polyomaviruses circulating within the human population that are genetically and serologically related to existing chimpanzee polyomaviruses.

【 授权许可】

CC BY