Estimating the Fitness Advantage Conferred by Permissive Neuraminidase Mutations in Recent Oseltamivir-Resistant A(H1N1)pdm09 Influenza Viruses

PLoS Pathogens

Lucia Reh¹  Sebastian Maurer-Stroh¹  Kathryn A. Hooper²  Raphael Lee³  Jesse D. Bloom³  Stephen Petrie⁴  Jodie McVernon⁵  Aeron C. Hurt⁶  Teagan Guarnaccia⁷  Chantal Baas⁸  Jeff Butler⁹  James M. McCaw⁹  Sook-Kwan Leang⁹  Sophie Vitesnik⁹  Lumin Xue⁹  Anne Kelso⁹  Ian G. Barr⁹ 

[1] Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR), Singapore;Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America;Melbourne School of Population and Global Health, The University of Melbourne, Parkville, Australia;Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, United States of America;Monash University, School of Applied Sciences, Churchill, Victoria, Australia;Murdoch Childrens Research Institute, The Royal Children's Hospital, Melbourne, Victoria, Australia;National Public Health Laboratory, Communicable Diseases Division Ministry of Health, Singapore;School of Biological Sciences (SBS), Nanyang Technological University (NTU), Singapore;World Health Organization Collaborating Centre for Reference and Research on Influenza, North Melbourne, Australia

关键词: Ferrets;    Microbial mutation;    Viral replication;    Influenza viruses;    Influenza;    Antimicrobial resistance;    Reverse transcriptase-polymerase chain reaction;    Viral transmission;    infection;   

DOI  :  10.1371/journal.ppat.1004065

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Oseltamivir is relied upon worldwide as the drug of choice for the treatment of human influenza infection. Surveillance for oseltamivir resistance is routinely performed to ensure the ongoing efficacy of oseltamivir against circulating viruses. Since the emergence of the pandemic 2009 A(H1N1) influenza virus (A(H1N1)pdm09), the proportion of A(H1N1)pdm09 viruses that are oseltamivir resistant (OR) has generally been low. However, a cluster of OR A(H1N1)pdm09 viruses, encoding the neuraminidase (NA) H275Y oseltamivir resistance mutation, was detected in Australia in 2011 amongst community patients that had not been treated with oseltamivir. Here we combine a competitive mixtures ferret model of influenza infection with a mathematical model to assess the fitness, both within and between hosts, of recent OR A(H1N1)pdm09 viruses. In conjunction with data from in vitro analyses of NA expression and activity we demonstrate that contemporary A(H1N1)pdm09 viruses are now more capable of acquiring H275Y without compromising their fitness, than earlier A(H1N1)pdm09 viruses circulating in 2009. Furthermore, using reverse engineered viruses we demonstrate that a pair of permissive secondary NA mutations, V241I and N369K, confers robust fitness on recent H275Y A(H1N1)pdm09 viruses, which correlated with enhanced surface expression and enzymatic activity of the A(H1N1)pdm09 NA protein. These permissive mutations first emerged in 2010 and are now present in almost all circulating A(H1N1)pdm09 viruses. Our findings suggest that recent A(H1N1)pdm09 viruses are now more permissive to the acquisition of H275Y than earlier A(H1N1)pdm09 viruses, increasing the risk that OR A(H1N1)pdm09 will emerge and spread worldwide.

【 授权许可】

CC BY