A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa

PLoS Pathogens

Jean-Jacques Muyembe¹  Taylor Sittler¹  Anne W. Rimoin²  Jonna Mazet³  Eric Delwart⁴  Nathan D. Wolfe⁵  Elizabeth Slikas⁶  J. Graham Ruby⁶  Maria Makuwa⁶  Graham Simmons⁷  Charles Y. Chiu⁸  Deanna Lee⁸  Eric M. Leroy⁸  Robert B. Tesh⁹  Travis Taylor^1,10  Narayanan Veeraraghavan^1,10  Bradley S. Schneider^1,10  Joseph N. Fair^1,11  Prime Mulembakani^1,12  Imke Steffen^1,12  Chunlin Wang^1,12  Gilda Grard^1,13 

[1] Blood Systems Research Institute, San Francisco, California, United States of America;Department of Biochemistry, Stanford University, Stanford, California, United States of America;Department of Biochemistry, University of California, San Francisco, California, United States of America;Department of Epidemiology, University of California at Davis, Davis, California, United States of America;Department of Epidemiology, University of California at Los Angeles, Los Angeles, California, United States of America;Department of Laboratory Medicine, University of California, San Francisco, California, United States of America;Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America;Global Viral Forecasting, Incorporated, San Francisco, California, United States of America;Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America;Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo;MIVEGEC, UMR (IRD 224 - CNRS 5290 - UM1 - UM2), Institut de Recherche pour le Développement, Montpellier, France;UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California, United States of America;Viral Emergent Diseases unit, Centre International de Recherches Médicales de Franceville, Franceville, Gabon

关键词: Hemorrhagic fevers;    Rhabdoviruses;    Phylogenetic analysis;    Viral hemorrhagic fevers;    Vesicular stomatitis virus;    Hemorrhagic fever viruses;    Polymerase chain reaction;    Ebola virus;   

DOI  :  10.1371/journal.ppat.1002924

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, mucosal hemorrhage, and, in two patients, death within 3 days. BASV was detected in an acute serum sample from the lone survivor at a concentration of 1.09×106 RNA copies/mL, and 98.2% of the genome was subsequently de novo assembled from ∼140 million sequence reads. Phylogenetic analysis revealed that BASV is highly divergent and shares less than 34% amino acid identity with any other rhabdovirus. High convalescent neutralizing antibody titers of >1∶1000 were detected in the survivor and an asymptomatic nurse directly caring for him, both of whom were health care workers, suggesting the potential for human-to-human transmission of BASV. The natural animal reservoir host or arthropod vector and precise mode of transmission for the virus remain unclear. BASV is an emerging human pathogen associated with acute hemorrhagic fever in Africa.

【 授权许可】

CC BY