Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

Biological research: BR

Masahiko Yoneda¹  Masahiko Gosho²  Masayuki Takeyama³  Masahiro Zako³  Masayoshi Iwaki³ 

[1] Department of Biochemistry and Molecular Biology, School of Nursing and Health, Aichi Prefectural University, Nagoya, Japan;Department of Clinical Trial and Clinical Epidemiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan;Department of Ophthalmology, Aichi Medical University, Nagakute, Japan

关键词: Age-related macular degeneration (AMD);    Hypothermia;    Pigment epithelium–derived factor (PEDF);    Retinal pigment epithelium (RPE);    Vascular endothelial growth factor (VEGF);    Vitrectomy;   

DOI  :  10.1186/s40659-015-0034-7

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogenesis in retinal pigment epithelium (RPE). We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium–derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased. RPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.

【 授权许可】

CC BY