In vitro screening and evaluation of antivenom phytochemicals from Azima tetracantha Lam. leaves against Bungarus caeruleus and Vipera russelli

Journal of venomous animals and toxins

Bhavya Janardhan¹  Vineetha M Shrikanth¹  Sunil S More¹  Kiran K Mirajkar² 

[1] Department of Biochemistry, Centre for Post Graduate Studies, Jain University, Bangalore, India;Department of Biochemistry, University of Agricultural Sciences, Dharwad, India

关键词: Acetylcholinesterase;    Azima tetracantha;    Antivenom;    Krait;    Viper;    In vitro;   

DOI  :  10.1186/1678-9199-20-12

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. Although antivenom is the only treatment available, it is associated with several side effects. As an alternative, plants have been extensively studied in order to obtain an alternative treatment. In folk medicine, Azima tetracantha Lam. is usually used to treat snakebites. The present study aims to provide a scientific explanation for the use of this plant against snakebite. The extracts of shade dried leaves of A. tetracantha were tested for in vitro inhibitory activity on toxic venom enzymes like phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase etc. from Bungarus caeruleus and Vipera russelli venoms. The ethylacetate extract rendered a significant inhibitory effect on the phosphomonoesterase, phosphodiesterase, phospholipase A2 and acetylcholinesterase enzymes. The present study suggests that ethylacetate extract of A. tetracantha leaves possesses compounds that inhibit the activity of toxic enzymes from Bungarus caeruleus and Vipera russelli venom. Further pharmacological and in vivo studies would provide evidence that this substance may lead to a potential treatment against these venoms.

【 授权许可】

CC BY