Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO

PLoS Pathogens

Aiming Wang¹  Xueping Zhou²  Kristin D. Kasschau³  Ruyi Xiong⁴  Changwei Zhang⁴  James C. Carrington⁵  Taiyun Wei⁵  Jian Hong⁵ 

[1] Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, United States of America;Center of Analysis and Measurement, Zhejiang University, Hangzhou, People's Republic of China;College of Horticulture, Nanjing Agricultural University, Nanjing, People's Republic of China;Department of Biology, The University of Western Ontario, London, Ontario, Canada;Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada

关键词: Viral structure;    Cytoplasm;    Leaves;    Myosins;    Plant viral pathogens;    Secretory pathway;    Plant cell walls;    Pathogen motility;   

DOI  :  10.1371/journal.ppat.1000962

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Intercellular transport of viruses through cytoplasmic connections, termed plasmodesmata (PD), is essential for systemic infection in plants by viruses. Previous genetic and ultrastructural data revealed that the potyvirus cyclindrical inclusion (CI) protein is directly involved in cell-to-cell movement, likely through the formation of conical structures anchored to and extended through PD. In this study, we demonstrate that plasmodesmatal localization of CI in N. benthamiana leaf cells is modulated by the recently discovered potyviral protein, P3N-PIPO, in a CI:P3N-PIPO ratio-dependent manner. We show that P3N-PIPO is a PD-located protein that physically interacts with CI in planta. The early secretory pathway, rather than the actomyosin motility system, is required for the delivery of P3N-PIPO and CI to PD. Moreover, CI mutations that disrupt virus cell-to-cell movement compromise PD-localization capacity. These data suggest that the CI and P3N-PIPO complex coordinates the formation of PD-associated structures that facilitate the intercellular movement of potyviruses in infected plants.

【 授权许可】

CC BY