Transcriptomic Analysis Implicates the p53 Signaling Pathway in the Establishment of HIV-1 Latency in Central Memory CD4 T Cells in an In Vitro Model

PLoS Pathogens

Nadejda Beliakova-Bethell¹  Celsa A. Spina²  Vicente Planelles³  David M. Margolis⁴  Cory H. White⁵  Douglas D. Richman⁶  Laura J. Martins⁶  Christopher H. Woelk⁶  Bastiaan Moesker⁶  Alberto Bosque⁷ 

[1] Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, Hampshire, United Kingdom;Department of Medicine, University of California San Diego, La Jolla, California, United States of America;Department of Pathology, University of California San Diego, La Jolla, California, United States of America;Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, United States of America;Graduate Program in Bioinformatics and Systems Biology, University of California San Diego, La Jolla, California, United States of America;San Diego VA medical Center and Veterans Medical Research Foundation, San Diego, California, United States of America;UNC HIV Cure Center, Department of Medicine, Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America

关键词: HIV-1;    Gene expression;    Viral persistence;    latency;    T helper cells;    Viral replication;    HIV;    Viral gene expression;    Antiretroviral therapy;   

DOI  :  10.1371/journal.ppat.1006026

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The search for an HIV-1 cure has been greatly hindered by the presence of a viral reservoir that persists despite antiretroviral therapy (ART). Studies of HIV-1 latency in vivo are also complicated by the low proportion of latently infected cells in HIV-1 infected individuals. A number of models of HIV-1 latency have been developed to examine the signaling pathways and viral determinants of latency and reactivation. A primary cell model of HIV-1 latency, which incorporates the generation of primary central memory CD4 T cells (TCM), full-length virus infection (HIVNL4-3) and ART to suppress virus replication, was used to investigate the establishment of HIV latency using RNA-Seq. Initially, an investigation of host and viral gene expression in the resting and activated states of this model indicated that the resting condition was reflective of a latent state. Then, a comparison of the host transcriptome between the uninfected and latently infected conditions of this model identified 826 differentially expressed genes, many of which were related to p53 signaling. Inhibition of the transcriptional activity of p53 by pifithrin-α during HIV-1 infection reduced the ability of HIV-1 to be reactivated from its latent state by an unknown mechanism. In conclusion, this model may be used to screen latency reversing agents utilized in shock and kill approaches to cure HIV, to search for cellular markers of latency, and to understand the mechanisms by which HIV-1 establishes latency.

【 授权许可】

CC BY