pelo Is Required for High Efficiency Viral Replication

PLoS Pathogens

Chuan-Qi Zhong¹  Xiurong Wu¹  Lisheng Li¹  Yuanyue Li¹  Jiahuai Han¹  Wanze Chen¹  Wan-Ting He¹  Dan Meng¹  Shuye Tian¹  Lili Tian¹  Felicia Han¹  Jianming Chen² 

[1] State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China;The Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration of China, Xiamen, Fujian, China

关键词: Ribosomes;    Drosophila melanogaster;    Viral replication;    Viral packaging;    RNA extraction;    RNA synthesis;    Luciferase;    Protein synthesis;   

DOI  :  10.1371/journal.ppat.1004034

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Viruses hijack host factors for their high speed protein synthesis, but information about these factors is largely unknown. In searching for genes that are involved in viral replication, we carried out a forward genetic screen for Drosophila mutants that are more resistant or sensitive to Drosophila C virus (DCV) infection-caused death, and found a virus-resistant line in which the expression of pelo gene was deficient. Our mechanistic studies excluded the viral resistance of pelo deficient flies resulting from the known Drosophila anti-viral pathways, and revealed that pelo deficiency limits the high level synthesis of the DCV capsid proteins but has no or very little effect on the expression of some other viral proteins, bulk cellular proteins, and transfected exogenous genes. The restriction of replication of other types of viruses in pelo deficient flies was also observed, suggesting pelo is required for high level production of capsids of all kinds of viruses. We show that both pelo deficiency and high level DCV protein synthesis increase aberrant 80S ribosomes, and propose that the preferential requirement of pelo for high level synthesis of viral capsids is at least partly due to the role of pelo in dissociation of stalled 80S ribosomes and clearance of aberrant viral RNA and proteins. Our data demonstrated that pelo is a host factor that is required for high efficiency translation of viral capsids and targeting pelo could be a strategy for general inhibition of viral infection.

【 授权许可】

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