Apoptosis in HEp-2 cells infected with Ureaplasma diversum

Biological research: BR

Aline Teixeira Amorim¹  Maysa Santos Barbosa¹  Jorge Timenetsky²  ssia Neves Lobã²  Ewerton Ferraz Andrade³  Angelita Maria Oliveira Gusmã³  Lucas Miranda Marques⁴  Izadora Souza Rezende⁴  Guilherme Barreto Campos⁵  o Santos⁵  Beatriz Araujo Cortez⁷  o⁸  Glaucia Maria Machado-Santelli⁹  Telma Alvez Monezi^1,10  Hellen Braga Martins^1,11  Tá^1,11 

[1] Instituto Multidisciplinar em SaúInstituto de Ciêcleo de Tecnologia em Saúde, Núde, Universidade Federal da Bahia, Vitódicas, Departamento de Biologia Celular e do Desenvolvimento, Universidade de Sãdicas, Departamento de Microbiologia, Universidade de Sãncias Bioméo Paulo, Brazil;o Paulo, Sãria da Conquista, Brazil

关键词: Ureaplasma diversum;    Invasion;    Apoptosis;    HEp-2 cells;   

DOI  :  10.1186/0717-6287-47-38

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location of Ureaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversum invasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.

【 授权许可】

CC BY