Seroepidemiological analysis of toxoplasmosis in college students

Journal of venomous animals and toxins

Monica Yonashiro Marcelino¹  Carolina Arruda de Faria¹  Heitor Franco de Andrade-Junior³  Marislene Santos⁴  Jaqueline Polizeli Rodrigues⁵  Italmar Teodorico Navarro⁷  Joã⁸  o Tadeu Ribeiro-Paes⁹  Luciana Pereira Silva⁹  Fernando Frei^1,10 

[1] Department of Biological Sciences, (UNESP), Assis, Brazil;Department of Genetics, RibeirãDepartment of Veterinary Medicine, Londrina State University, Londrina, Brazil;Graduate Program in Biotechnology, University of SãInstitute of Tropical Medicine of São Paulo (USP), Ribeirão Paulo (USP), São Paulo (USP)/Butantan Institute/Institute for Technological Research (IPT), São Paulo, Brazil;o Paulo, University of São Preto Medical School, University of São Preto, Brazil

关键词: Toxoplasma gondii;    Toxoplasmosis;    Enzyme-linked immunosorbent assay;    Indirect fluorescent antibody test;    Seroprevalence;    Epidemiology;   

DOI  :  10.1186/1678-9199-21-1

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondii antibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.

【 授权许可】

CC BY