Set-up of a method using LC-UV to assay mometasone furoate in pharmaceutical dosage forms

Quimica nova

Melero, Ana¹  Schaefer, Ulrich F.¹  Ourique, Aline Ferreira²  Beck, Ruy Carlos Ruver²  Contri, Renata Vidor²  Pohlmann, Adriana Raffin²  Guterres, Silvia Stanisçuaski² 

[1] Saarland University, Saarbrücken, Germany;Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil

关键词: pharmaceuticals;    liquid chromatography;    mometasone.;   

DOI  :  10.1590/S0100-40422012000400030

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A reliable method using LC-UV to assay mometasone furoate (MF) in creams or nasal sprays using the same chromatographic conditions was set up. Methanol:water 80:20 (v/v) (1.0 mL min-1) was used as mobile phase. MF was detected at 248 nm and analyzed in a concentration range from 1.0 to 20.0 µg mL-1. The method provided acceptable theoretical plates, peak simmetry, peak tailing factor and peak resolution a short run (5 min). The method showed specificity, good linearity (r = 0.9999) and the quantification limit was 0.379 µg mL-1. Furthermore, the method was precise (RSD < 2.0%), accurate (recovery > 97%) and robust.

【 授权许可】

CC BY