Historical Contingencies Modulate the Adaptability of Rice Yellow Mottle Virus

PLoS Pathogens

Alain Ghesquière¹  Florence Vignols²  Denis Fargette²  Nils Poulicard²  Agnès Pinel-Galzi²  Eugénie Hébrard²  Gnissa Konaté³  Oumar Traoré³ 

[1] Institut de Recherche pour la Développement (IRD), UMR DIADE (IRD, CIRAD, Université Montpellier 2), Montpellier, France;Institut de Recherche pour le Développement (IRD), UMR RPB (IRD, CIRAD, Université Montpellier 2), Montpellier, France;Institut de l'Environnement et de Recherches Agricoles (INERA), Ouagadougou, Burkina-Faso

关键词: Rice;    Glutamic acid;    Genetic determinism;    Threonine;    Point mutation;    Viral evolution;    Mutagenesis;    Microbial mutation;   

DOI  :  10.1371/journal.ppat.1002482

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The rymv1-2 and rymv1-3 alleles of the RYMV1 resistance to Rice yellow mottle virus (RYMV), coded by an eIF(iso)4G1 gene, occur in a few cultivars of the Asiatic (Oryza sativa) and African (O. glaberrima) rice species, respectively. The most salient feature of the resistance breaking (RB) process is the converse genetic barrier to rymv1-2 and rymv1-3 resistance breakdown. This specificity is modulated by the amino acid (glutamic acid vs. threonine) at codon 49 of the Viral Protein genome-linked (VPg), a position which is adjacent to the virulence codons 48 and 52. Isolates with a glutamic acid (E) do not overcome rymv1-3 whereas those with a threonine (T) rarely overcome rymv1-2. We found that isolates with T49 had a strong selective advantage over isolates with E49 in O. glaberrima susceptible cultivars. This explains the fixation of the mutation T49 during RYMV evolution and accounts for the diversifying selection estimated at codon 49. Better adapted to O. glaberrima, isolates with T49 are also more prone than isolates with E49 to fix rymv1-3 RB mutations at codon 52 in resistant O. glaberrima cultivars. However, subsequent genetic constraints impaired the ability of isolates with T49 to fix rymv1-2 RB mutations at codons 48 and 52 in resistant O. sativa cultivars. The origin and role of the amino acid at codon 49 of the VPg exemplifies the importance of historical contingencies in the ability of RYMV to overcome RYMV1 resistance.

【 授权许可】

CC BY