The application of multiplex fluorimetric sensor for the analysis of flavonoids content in the medicinal herbs family Asteraceae, Lamiaceae, Rosaceae

Biological research: BR

Marek Zivcak¹  Elena Hunkova¹  Klaudia Bruckova¹  Kiessoun Konate¹  Marian Brestic²  Oksana Sytar³ 

[1] Department of Plant Physiology, Slovak University of Agriculture in Nitra, Nitra, Slovakia;Laboratory of Biochemistry and Applied Chemistry, University of Ouagadougou, Ouagadougou 09, Burkina Faso;Plant Physiology and Ecology Department, Institute of Biology, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

关键词: Non-destructive measurement;    Flavonoids;    Multiplex fluorimetric sensor;    Asteraceae;    Lamiaceae;    Rosaceae;   

DOI  :  10.1186/0717-6287-48-5

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

The aim of our research work was to quantify total flavonoid contents in the leaves of 13 plant species family Asteraceae, 8 representatives of family Lamiaceae and 9 plant species belonging to family Rosaceae, using the multiplex fluorimetric sensor. Fluorescence was measured using optical fluorescence apparatus Multiplex(R) 3 (Force-A, France) for non-destructive flavonoids estimation. The content of total flavonoids was estimated by FLAV index (expressed in relative units), that is deduced from flavonoids UV absorbing properties. Among observed plant species, the highest amount of total flavonoids has been found in leaves of Helianthus multiflorus (1.65 RU) and Echinops ritro (1.27 RU), Rudbeckia fulgida (1.13 RU) belonging to the family Asteraceae. Lowest flavonoid content has been observed in the leaves of marigold (Calendula officinalis) (0.14 RU) also belonging to family Asteraceae. The highest content of flavonoids among experimental plants of family Rosaceae has been estimated in the leaves of Rosa canina (1.18 RU) and among plant species of family Lamiaceae in the leaves of Coleus blumei (0.90 RU). This research work was done as pre-screening of flavonoids content in the leaves of plant species belonging to family Asteraceae, Lamiaceae and Rosaceae. Results indicated that statistically significant differences (P > 0.05) in flavonoids content were observed not only between families, but also among individual plant species within one family.

【 授权许可】

CC BY