MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae

PLoS Pathogens

Ping Wang¹  Xiao Li²  Xinyi Le²  Haifeng Zhang²  Kaili Zhong²  Xiaobo Zheng²  Xiangyi Kong²  Zhengguang Zhang² 

[1] Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America;Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China

关键词: Mitochondria;    Peroxisomes;    Appressoria;    Fluorescence microscopy;    Autophagic cell death;    Deletion mutagenesis;    Pathogenesis;    Rice;   

DOI  :  10.1371/journal.ppat.1005823

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Dynamins are large superfamily GTPase proteins that are involved in various cellular processes including budding of transport vesicles, division of organelles, cytokinesis, and pathogen resistance. Here, we characterized several dynamin-related proteins from the rice blast fungus Magnaporthe oryzae and found that MoDnm1 is required for normal functions, including vegetative growth, conidiogenesis, and full pathogenicity. In addition, we found that MoDnm1 co-localizes with peroxisomes and mitochondria, which is consistent with the conserved role of dynamin proteins. Importantly, MoDnm1-dependent peroxisomal and mitochondrial fission involves functions of mitochondrial fission protein MoFis1 and WD-40 repeat protein MoMdv1. These two proteins display similar cellular functions and subcellular localizations as MoDnm1, and are also required for full pathogenicity. Further studies showed that MoDnm1, MoFis1 and MoMdv1 are in complex to regulate not only peroxisomal and mitochondrial fission, pexophagy and mitophagy progression, but also appressorium function and host penetration. In summary, our studies provide new insights into how MoDnm1 interacts with its partner proteins to mediate peroxisomal and mitochondrial functions and how such regulatory events may link to differentiation and pathogenicity in the rice blast fungus.

【 授权许可】

CC BY