ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT

Journal of The Chilean Chemical Society

RANE, VIPUL. P¹  PATIL, KIRAN. R¹  SHINDE, DEVANAND. B¹  YEOLE, RAVINDRA. D¹ 

[1] United Arab Emirates University, Aurangabad, India

关键词: Metolazone;    Method development;    Enantiomeric separation;    Amylose based stationary phase;    Validation.;   

DOI  :  10.4067/S0717-97072013000100019

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

A simple, rapid and robust LC method was developed and validated for the enantiomeric separation of metolazone. The enantiomers of metolazone were resolved on a Chiralpak AD-H (amylose based stationary phase) column using a mobile phase consisting of Hexane: 2-propanol: Methanol: Acetic acid (80:10:10:0.2, v/v) at a flow rate of 1.0 mLmin-1. The resolution between the enantiomers was found to be not less than 3.0 in optimized method. The presence of acetic acid in the mobile phase played an important role, in enhancing chromatographic efficiency and resolution between the enantiomers. The developed method was extensively validated and proved to be robust.

【 授权许可】

Unknown