Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

Biological research: BR

Yang Wang¹  Xianqiong Zou²  Ruixin Li²  Yong Guo²  Lu Liu³  Xinchang Zhang³  Yongming Liu³  Qiangcheng Zeng⁴  Xizheng Zhang⁵ 

[1] Chemistry Department, Logistics College of Chinese People’College of Biotechnology, Guilin Medical University, Guilin, China;Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, China;Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Institute of Biophysics, Dezhou University, Dezhou, China;s Armed Police Forces, Tianjin, China

关键词: Tensile strain;    Osteoblast;    Extracellular matrix;    Integrin;    siRNA;   

DOI  :  10.1186/s40659-015-0014-y

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (με) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by the strain. At the same time, the result of ECM formation promoted by the strain, was similar to the osteoblastic differentiation. Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.

【 授权许可】

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