Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential

BioMedical Engineering OnLine

Yong Guo³  Chun-qiu Zhang²  Qiang-cheng Zeng¹  Rui-xin Li³  Lu Liu³  Qin-xin Hao³  Cai-hong Shi³  Xi-zheng Zhang³  Yu-xian Yan³ 

[1] Shandong provincial Key lab of biophysics, Dezhou University, No. 566 Daxuexi Road, Decheng District, Dezhou, 253021, China

[2] School of Mechanical Engineering, Tianjin University of Technology, No. 263 Hongqi Nalu Road ,Nankai District, Tianjin, 300191, China

[3] Academy of Military Medical Science, Tianjin Institute of Medical Equipment, No 106 Wandong Road, Hedong District, Tianjin, 300161, China

关键词: Osteoinduction;    Extracellular matrix;    Osteoblast;    Tensile strain;   

Others  :  797992
DOI  :  10.1186/1475-925X-11-80

 received in 2012-06-03, accepted in 2012-10-09,  发布年份 2012

【 摘 要 】

Background

The extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity.

Methods

Mouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM.

Results

The cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells.

Conclusion

Mechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts.

【 授权许可】

   
2012 Guo et al.; licensee BioMed Central Ltd.

【 图 表 】

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