Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants

Quimica nova

Cunha Junior, Armando da Silva¹  Saliba, Juliana Barbosa¹  Mansur, Herman Sander¹  Gomes, Elionai Cassiana de Lima¹  Silva, Gisele Rodrigues da² 

[1] Universidade Federal de Minas Gerais, Belo Horizonte, Brasil;Universidade Federal de São João Del Rei, Divinópolis, Brasil

关键词: cyclosporine-A;    biodegradable intraocular implant;    validation.;   

DOI  :  10.1590/S0100-40422011000100026

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

An HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase, a C18 column at 80 ºC and UV detection at 210 nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 2.5-40.0 µg/mL. The quantitation and detection limits were 0.8 and 1.2 µg/mL, respectively. The recovery was 101.8% and intra-day and inter-day precision was close to 2%.

【 授权许可】

CC BY