Comparative performance of Candida rugosa lipase immobilized on polysiloxane polyvinyl alcohol hybrid support using different methodologies

Quimica nova

Bruno, Laura M.¹  Braga, Luciana P.²  Moreira, Ana B. R.²  Paula, Ariela V.²  Castro, Heizir F. de² 

[1] Embrapa Agroindústria Tropical, Fortaleza, Brasil;Universidade de São Paulo, Lorena, Brasil

关键词: lipase;    hybrid support;    adsorption.;   

DOI  :  10.1590/S0100-40422008000100007

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The efficiency for immobilizing microbial Candidarugosa lipase on a hybrid matrix of polysiloxane polyvinyl alcohol, by adsorption,covalent coupling and encapsulation was compared. The activities of immobilizedderivatives were evaluated using p-nitrophenylpalmitate (hydrolysis)and butyric acid and butanol (esterification) as substrates. Operational stabilityand storage tests were also performed. Among the procedures tested, the proposedmatrix was efficient for immobilizing C. rugosa lipase by adsorptionand covalent coupling techniques and unsuitable for encapsulation purposes.The results reveal that better catalytic properties in both aqueous and organicmedia were demonstrated by the covalent coupling POS-PVA immobilized lipase,including also satisfactory half-life and good storage stability.

【 授权许可】

CC BY