Comparação do desempenho da lipase de candida rugosa imobilizada em suporte híbrido de polissiloxano-polivinilálcool empregando diferentes metodologias

Química Nova

Ariela V. Paula²  Ana B. R. Moreira²  Luciana P. Braga²  Heizir F. De Castro²  Laura M. Bruno¹ 

[1] ,Universidade de São Paulo Escola de Engenharia de Lorena Lorena SP ,Brasil

关键词: lipase;    hybrid support;    adsorption;   

DOI  :  10.1590/S0100-40422008000100007

来源: SciELO

【 摘 要 】

The efficiency for immobilizing microbial Candida rugosa lipase on a hybrid matrix of polysiloxane polyvinyl alcohol, by adsorption, covalent coupling and encapsulation was compared. The activities of immobilized derivatives were evaluated using p-nitrophenylpalmitate (hydrolysis) and butyric acid and butanol (esterification) as substrates. Operational stability and storage tests were also performed. Among the procedures tested, the proposed matrix was efficient for immobilizing C. rugosa lipase by adsorption and covalent coupling techniques and unsuitable for encapsulation purposes. The results reveal that better catalytic properties in both aqueous and organic media were demonstrated by the covalent coupling POS-PVA immobilized lipase, including also satisfactory half-life and good storage stability.

【 授权许可】

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