(Homo)glutathione Deficiency Impairs Root-knot Nematode Development in Medicago truncatula

PLoS Pathogens

Bruno Favery¹  Philippe Lecomte¹  Pierre Abad¹  Fabien Baldacci-Cresp²  Stéphane Bernillon²  Didier Hérouart²  Pierre Frendo²  Christine Chang²  Renaud Brouquisse²  Annick Moing³  Catherine Deborde³  Alain Puppo³  Julie Hopkins³  Mickaël Maucourt⁴ 

[1] INRA - UMR 1332 Biologie du Fruit et Pathologie, Centre INRA de Bordeaux, Villenave d'Ornon, France;Interactions Biotiques et Santé Végétale UMR INRA 1301 -CNRS 6243-Université de Nice-Sophia Antipolis, Sophia Antipolis, France;Metabolome-Fluxome Facility of Bordeaux Functional Genomics Center, IBVM, Centre INRA de Bordeaux, Villenave d'Ornon, France;Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, Centre INRA de Bordeaux, Villenave d'Ornon, France

关键词: Nematode infections;    Glucose metabolism;    Metabolites;    Starches;    Gene expression;    Nitrogen metabolism;    Glutathione;    Mass production;   

DOI  :  10.1371/journal.ppat.1002471

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Root-knot nematodes (RKN) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, RKN induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells essential for nematode growth and reproduction. These metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. Detailed analysis of glutathione (GSH) and homoglutathione (hGSH) metabolism demonstrated the importance of these compounds for the success of nematode infection in Medicago truncatula. We reported quantification of GSH and hGSH and gene expression analysis showing that (h)GSH metabolism in neoformed gall organs differs from that in uninfected roots. Depletion of (h)GSH content impaired nematode egg mass formation and modified the sex ratio. In addition, gene expression and metabolomic analyses showed a substantial modification of starch and γ-aminobutyrate metabolism and of malate and glucose content in (h)GSH-depleted galls. Interestingly, these modifications did not occur in (h)GSH-depleted roots. These various results suggest that (h)GSH have a key role in the regulation of giant cell metabolism. The discovery of these specific plant regulatory elements could lead to the development of new pest management strategies against nematodes.

【 授权许可】

CC BY