Interaction of the extracts of three medicinal plants with antibiotics against some antibiotic resistant bacteria

Scientific Research and Essays

Eze¹ 

关键词:  ;    Medicinal plants;    antibiotics;    antibiotic-extract combination;    multidrug-resistant bacteria.;   

DOI  :  10.5897/SREX2013.5515

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

The outcome ofin vitrointeraction of standard antibiotics with ethanolic leaf extracts of three medicinal plants (Picralima nitida, Chromolaena odorataandAspilia africana) against antibiotic resistant bacteria was investigated by agar disc diffusion methods and macrobroth dilution techniques. At least three isolates each ofEscherichia coli, Staphylococcus aureusandPseudomonas aeruginosathat were resistant to more than two of the following antibiotic discs were used: erythromycin, chloramphenicol, tetracycline, norfloxacin and ciprofloxacin. A subinhibitory concentration (1/4th MIC) of the plant extracts were combined with the antibiotics against the resistant bacteria isolates using both antibiotic disc diffusion technique and macrobroth dilution test. The extract ofA. africanaenhanced the activities of the test antibiotics (except erythromycin) against resistantE.coli, and also enhanced the activities of ciprofloxacin, norfloxacin and chloramphenicol against antibiotic resistantP. aeruginosa. It also enhanced the activities of tetracycline and norfloxacin against antibiotic resistantS. aureus.The extract ofC. odoratagreatly enhanced the activities of the antibiotics (except erythromycin) against the resistantP. aeruginosaand also potentiated the activities of chloramphenicol and tetracycline against resistantS. aureus. Although the extract ofP. nitidapotentiated few antibiotics such as tetracycline and chloramphenicol against resistantE. coli, the extract exhibited high levels of antagonism with ciprofloxacin and norfloxacin against almost all the test bacteria. These results suggest that extracts ofC.odorataandA.africanacould be good sources of multidrug resistance inhibitors, and indicate that indiscriminate co-administration of antibiotics with some herbal drugs such as those fromP. nitidacould be therapeutically wasteful. Their use in combination with conventional antibiotics should be further studied forin vivoactivities. This may lead to the development of much needed drug enhancing preparations.

【 授权许可】

CC BY