ULTRASOUND-ASSISTED EXTRACTION AND CHARACTERIZATION OF POLYSACCHARIDE FROM MARYLAND TOBACCO LEAVES

Journal of The Chilean Chemical Society

TIAN, ZHAOFU¹  LI, XIAOLAN¹  LIU, SHAOHUA¹  XU, CHUNPING²  HE, PEIXIN² 

[1] China Tobacco Guangxi Industrial Co. Ltd, Nanning, China;Zhengzhou University of Light Industry, Henan, China

关键词: antioxidant activity;    extraction;    optimization;    Maryland tobacco leaves;    polysaccharide;   

DOI  :  10.4067/S0717-97072015000100001

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

Ultrasound-assisted extraction (UAE) of polysaccharide from Maryland tobacco leaves was studied by response surface methodology. Furthermore, the crude polysaccharide was purified and two components (Fr-I and Fr-II) were obtained. FT-IR spectral analysis of the purified polysaccharide revealed prominent characteristic groups. The monosaccharide composition analysis by gas chromatography-mass spectrometry (GC/MS) indicated the main composition between Fr-I and Fr-II was different. Furthermore, thermo gravimetric analysis (TGA) indicated the degradation temperature (Td) of the Fr-I (241 oC) was higher than those of Fr-II (216 oC). Detected by the pyrolysis gas chromatography-mass spectrometry (py-GC/MS), it was found that the main kinds of pyrolysis products from both Fr-I and Fr-II were similar. Finally, On the basis of hydroxyl and DPPH radical scavenging assay, Fr-II has stronger antioxidant activities than Fr-I. The thermal behavior and antioxidant activity might be attributed to the configuration of the chemical compositions.

【 授权许可】

Unknown