Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts

Sensors

Ljiljana Stanojević¹  Mihajlo Stanković¹  Vesna Nikolić¹  Ljubiᘚ Nikolić¹  Dušica Ristić¹  Jasna ჊nadanovic-Brunet² 

[1] University of Nis, Faculty of Technology, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia; E-Mails:;University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia; E-Mails:

关键词: Hieracium pilosella L. (Asteraceae);    antioxidant activity;    extraction;    total phenolic content;    total flavonoids;    HPLC determination;   

DOI  :  10.3390/s90705702

来源: mdpi

【 摘 要 】

The antioxidant activity of water, ethanol and methanol Hieracium pilosella L. extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH^• free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH^• radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59–244.16 mg GAE/g of dry extract) and total flavonoids (79.13–82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity.

【 授权许可】

CC BY   
© 2009 by the authors; licensee MDPI, Basel, Switzerland