Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2

PLoS Pathogens

Binshan Shi¹  Paul Cristofano²  Carlos M. C. de Noronha³  Andrea Kinga Marias Furuya³  Hamayun J. Sharifi³  Robert M. Jellinger⁴ 

[1] Albany College of Pharmacy and Health Sciences, Albany, New York, United States of America;Albany Medical College, Albany Medical Center, Albany, New York, United States of America;Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York, United States of America;Division of HIV Medicine, Albany Medical Center, Albany, New York, United States of America

关键词: HIV-1;    Macrophages;    Luciferase;    HIV infections;    HIV;    Small interfering RNAs;    HIV-2;    T cells;   

DOI  :  10.1371/journal.ppat.1005581

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Marburg virus, the Kaposi's sarcoma-associated herpesvirus (KSHV) and Dengue virus all activate, and benefit from, expression of the transcription regulator nuclear erythroid 2-related factor 2 (Nrf2). The impact of Nrf2 activation on human immunodeficiency virus (HIV) infection has not been tested. Sulforaphane (SFN), produced in cruciferous vegetables after mechanical damage, mobilizes Nrf2 to potently reprogram cellular gene expression. Here we show for the first time that SFN blocks HIV infection in primary macrophages but not in primary T cells. Similarly SFN blocks infection in PMA-differentiated promonocytic cell lines, but not in other cell lines tested. siRNA-mediated depletion of Nrf2 boosted HIV infectivity in primary macrophages and reduced the anti-viral effects of SFN treatment. This supports a model in which anti-viral activity is mediated through Nrf2 after it is mobilized by SFN. We further found that, like the type I interferon-induced cellular anti-viral proteins SAMHD1 and MX2, SFN treatment blocks infection after entry, but before formation of 2-LTR circles. Interestingly however, neither SAMHD1 nor MX2 were upregulated. This shows for the first time that Nrf2 action can potently block HIV infection and highlights a novel way to trigger this inhibition.

【 授权许可】

CC BY