Technical Advance: Langerhans cells derived from a human cell line in a full‐thickness skin equivalent undergo allergen‐induced maturation and migration

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: MUTZ‐;    3;    in vitro;    keratinocyte;    fibroblast;    sensitization;    vaccination strategies;   

DOI  :  10.1189/jlb.0610374

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Inthisreport,theconstructionofafunctional,immunocompetent,full‐thicknessskinequivalent(SE)isdescribed,consistingofanepidermalcompartmentcontainingkeratinocytes,melanocytes,andhumanLCsderivedfromtheMUTZ‐3cellline(MUTZ‐LC)andafibroblast‐populateddermalcompartment.TheCD1a+Langerin+HLA‐DR+MUTZ‐LCspopulatetheentireepidermisatasimilardensitytothatfoundinnativeskin.ExposureoftheSEtosubtoxicconcentrationsoftheallergensNiSO4andresorcinolresultedinLCmigrationoutoftheepidermistowardthefibroblast‐populateddermalcompartment.Asignificantdose‐dependentup‐regulationoftheDCmaturation‐relatedCCR7andIL‐1βtranscriptsandofCD83attheproteinleveluponepidermalexposuretobothallergenswasobserved,indicativeofmaturationandmigrationoftheepidermallyincorporatedLC.Wehavethussuccessfullydevelopedareproducibleandfunctionalfull‐thicknessSEmodelcontainingepidermalMUTZ‐LC.Thismodeloffersanalternativetoanimaltestingforidentifyingpotentialchemicalsensitizersandforskin‐basedvaccinationstrategiesandprovidesauniqueresearchtooltostudyhumanLCbiologyinsituundercontrolledinvitroconditions...

【 授权许可】

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