Plastic restriction of HIV‐1 replication in human macrophages derived from M1/M2 polarized monocytes

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: APOBEC3A/3G;    DC‐;    SIGN;    CD4;    viral entry;    viral latency;   

DOI  :  10.1189/jlb.4AB0316-158R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

M1/M2cytokine‐dependentpolarizationofprimaryhumanMDMshasbeenshowntocontainCCR5‐dependent(R5)HIV‐1replication.Inthisstudy,asimilareffectwasachievedwhenmonocyteswerefirstpolarizedtowardM1orM2andwereinfected7daftertheirdifferentiationintoMDMs,regardlessofwhetherthecytokineswereremoved18haftercellstimulationorwereleftinculture.UnlikepolarizedMDMs,nosignificantdown‐regulationofCD4fromthecellsurfacewasobservedinMDMsderivedfromM1/M2‐polarizedmonocytes.AsecondstimulationofMDMsdifferentiatedfromM1/M2monocyteswiththeoppositepolarizingcytokinesconvertedthevirusreplicationprofileaccordingtothenewstimuli.TheexpressionofM1andM2markers(i.e.,APOBEC3AandDC‐SIGN,respectively)wasinducedbyMDMstimulationwiththeoppositecytokines,althoughitalsopersistedincellsaccordingtotheirfirststimulatorycondition.Thus,stimulationofmonocyteswithM1‐andM2‐inducingcytokinesleadstoarestrictionofHIV‐1replicationwhenthesecellsareinfectedseveraldayslaterasdifferentiatedMDMs.TheseobservationsimplythatactivationofcirculatingmonocytessignificantlyinfluencestheircapacitytoeithersupportorrestrictHIV‐1replication,onceextravasated,andeventuallytobecomeinfectedastissuemacrophages...

【 授权许可】

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