Mouse neutrophils express the decoy type 2 interleukin‐1 receptor (IL‐1R2) constitutively and in acute inflammatory conditions

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: granulocytes;    hydrocortisone;    LPS;    shedding;   

DOI  :  10.1189/jlb.0113035

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TheproinflammatoryactivitiesofIL‐1aretightlycontrolledatdifferentlevels.IL‐1R2actsasadecoyreceptorandhasbeenshowntoregulatethebiologicaleffectsofIL‐1invitroandinvivo.However,littleisknownaboutitsnaturalexpressioninthemouseinphysiologicandpathologicconditions.Inthisstudy,weexaminedIL‐1R2mRNAandproteinexpressioninisolatedcellsandtissuesinresponsetodifferentstimulatoryconditions.DataobtainedusingexvivoCD11b+Ly6G+peripheralbloodcellsandinvitro‐differentiatedCD11b+Ly6G+BMGindicatedthatneutrophilsarethemajorsourceofconstitutivelyexpressedIL‐1R2inthemouse.TheexpressionofIL‐1R2onBMGandexvivoLy6G+peripheralbloodcellswashighlyup‐regulatedbyHC.IL‐1R2pull‐downexperimentsshowedthatmouserIL‐1βbindstoBMGIL‐1R2,whereasbindingofIL‐1Racouldnotbedetected.Furthermore,LPStreatmentinducedsheddingofIL‐1R2fromtheneutrophilmembraneinvitroandinvivo,executedmainlybyADAM17.Finally,ininvivomodelsofinflammation,includingthioglycolate‐inducedacuteperitonitisandacutelunginjury,infiltratingLy6G+neutrophils,expressedIL‐1R2.Ourdatashowthatinthemouse,neutrophilsmainlyexpressthedecoyreceptorIL‐1R2undernaïveandinflammatoryconditions.Thesedatasuggestthatneutrophilsmaycontributetotheresolutionofacuteinflammation...

【 授权许可】

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