Use of quantitative real-time RT-PCR to investigate the correlation betweenviremia and viral shedding of canine distemper virus, and infection outcomes inexperimentally infected dogs

Journal of Veterinary Medical Science

Eiji OISHI¹  Go SEHATA¹  Yoshitaka IMAIZUMI¹  Taichi NORO¹  Toshihiro ITO¹  Hiroaki SATO¹ 

[1] Kyoto Biken Laboratories, Inc., 24�?16 Makishima, Uji, Kyoto, Japan

关键词: canine distemper;    kinetics;    real-time RT-PCR;    shedding;    viremia;   

DOI  :  10.1292/jvms.14-0066

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)We used real-time RT-PCR and virus titration to examine canine distemper virus(CDV) kinetics in peripheral blood and rectal and nasal secretions from 12 experimentallyinfected dogs. Real-time RT-PCR proved extremely sensitive, and the correlation betweenthe two methods for rectal and nasal (r=0.78, 0.80) samples on the peak day of viral RNAwas good. Although the dogs showed diverse symptoms, viral RNA kinetics were similar; thepeak of viral RNA in the symptomatic dogs was consistent with the onset of symptoms. Theseresults indicate that real-time RT-PCR is sufficiently sensitive to monitor CDVreplication in experimentally infected dogs regardless of the degree of clinicalmanifestation and suggest that the peak of viral RNA reflects active CDV replication.

【 授权许可】

Unknown