ATP-Sensitive Cation-channel in Wheat (Triticum durum Desf.): Identification and Characterization of a Plant Mitochondrial Channel by Patch-clamp

Cellular Physiology and Biochemistry

关键词: Mitochondria;    Patch clamp;    ATP-sensitive cation channel;    Plant;   

DOI  :  10.1159/000324010

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Indirect evidence points to the presence of K⁺ channels in plant mitochondria. In the present study, we report the results of the first patch clamp experiments on plant mitochondria. Single-channel recordings in 150 mM potassium gluconate have allowed the biophysical characterization of a channel with a conductance of 150 pS in the inner mitochondrial membrane of mitoplasts obtained from wheat (Triticum durum Desf.). The channel displayed sharp voltage sensitivity, permeability to potassium and cation selectivity. ATP in the mM concentration range completely abolished the activity. We discuss the possible molecular identity of the channel and its possible role in the defence mechanisms against oxidative stress in plants.

【 授权许可】

CC BY-NC-ND