Human MAIT‐cell responses to Escherichia coli: activation, cytokine production, proliferation, and cytotoxicity

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: MR1;    innate‐;    like T cells;    antimicrobial activity;    functional evaluation;   

DOI  :  10.1189/jlb.4TA0815-391RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Mucosa‐associatedinvariantTcellsarealargeandrelativelyrecentlydescribedinnate‐likeantimicrobialT‐cellsubsetinhumans.Thesecellsrecognizeriboflavinmetabolitesfromarangeofmicrobespresentedbyevolutionarilyconservedmajorhistocompatibilitycomplex,classI‐relatedmolecules.Giventheinnate‐likecharacteristicsofmucosa‐associatedinvariantTcellsandthenoveltypeofantigenstheyrecognize,newmethodologymustbedevelopedandexistingmethodsrefinedtoallowcomprehensivestudiesoftheirroleinhumanimmunedefenseagainstmicrobialinfection.Inthisstudy,weestablishedprotocolstoexaminearangeofmucosa‐associatedinvariantT‐cellfunctionsastheyrespondtoantigenproducedbyEscherichiacoli.Theseimprovedanddose‐andtime‐optimizedexperimentalprotocolsallowdetailedstudiesofMR1‐dependentmucosa‐associatedinvariantT‐cellresponsestoEscherichiacolipulsedantigen‐presentingcells,asassessedbyexpressionofactivationmarkersandcytokines,byproliferation,andbyinductionofapoptosisanddeathinmajorhistocompatibilitycomplex,classI‐related–expressingtargetcells.Thenovelandoptimizedprotocolsestablishaframeworkofmethodsandopennewpossibilitiestostudymucosa‐associatedinvariantT‐cellimmunobiology,usingEscherichiacoliasamodelantigen.Furthermore,weproposethattheserobustexperimentalsystemscanalsobeadaptedtostudymucosa‐associatedinvariantT‐cellresponsestoothermicrobesandtypesofantigen‐presentingcells...

【 授权许可】

CC BY