Effects of a polyphenol present in olive oil, oleuropein aglycone, in a murine model of intestinal ischemia/reperfusion injury

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: SAO shock;    inflammation;    ROS;    NF‐;    κ;    B;   

DOI  :  10.1189/jlb.0712317

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Dietaryoliveoilsupplementationandmorerecently,oliveoilphenolshavebeenrecommendedasimportanttherapeuticinterventionsinpreventivemedicine.Olehasseveralpharmacologicalproperties,includingantioxidant,anti‐inflammatory,antiatherogenic,anticancer,antimicrobial,andantiviralandforthesereasons,isbecominganimportantsubjectofstudyinrecentyears.TheaimofthisstudywastoinvestigatetheeffectsofOleaglyconeonthemodulationofthesecondaryeventsinmicesubjectedtointestinalIRI.Thiswasinducedinmicebyclampingthesuperiormesentericarteryandtheceliactrunkfor30min,followedbyreleaseoftheclamp,allowingreperfusionfor1h.After60minofreperfusion,animalswerekilledforhistologicalexaminationoftheileumtissueandimmunohistochemicallocalizationofproinflammatorycytokines(TNF‐αandIL‐1β)andadhesionmolecules(ICAM‐1andP‐sel);moreover,byWesternblotanalysis,weinvestigatedtheactivationofNF‐κBandIκBα.Inaddition,weevaluatedtheapoptosisprocess,asshownbyTUNELstainingandBax/Bcl‐2expressions.TheresultsobtainedbythehistologicalandmolecularexaminationsshowedinOleaglycone‐treatedmice,adecreaseofinflammationandapoptosispathwayversusSAO‐shockedmice.Inconclusion,weproposethattheoliveoilcompounds,inparticular,theOleaglycone,couldrepresentapossibletreatmentagainstsecondaryeventsofintestinalIRI...

【 授权许可】

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