Genospecies and virulence factors of Aeromonas species in different sources in a North African country

Libyan journal of medicine

Khalifa Sifaw Ghenghesh¹ 

关键词: Aeromonas;    genospecies;    virulence factors;    Libya;   

DOI  :  10.3402/ljm.v9.25497

学科分类：医学（综合）

来源: Co-Action Publishing

【 摘 要 】

IntroductionAeromonads of medical importance have been reported from numerous clinical, food, and water sources, but identification of genospecies and virulence factors of Aeromonas species from countries in North Africa and the Middle East are few.MethodsIn total 99 Aeromonas species isolates from different sources (diarrheal children [n=23], non-diarrheal children [n=16], untreated drinking water from wells [n=32], and chicken carcasses [n=28]) in Tripoli, Libya, were included in the present investigation. Genus identification was confirmed by biochemical analysis, and genospecies were determined using a combination of 16S rDNA variable region and gyrB sequence analysis. Polymerase chain reaction (PCR) was used to detect genes encoding toxins from 52 of the isolates.ResultsWe identified 44 isolates (44%) as A. hydrophila (3 [3.0%] subspecies anaerogenes, 23 [23%] subspecies dhakensis, and 18 [18%] subspecies ranae); 27 isolates (27%) as A. veronii; 23 isolates (23%) as A. caviae; and 5 isolates (5.0%) a...

【 授权许可】

CC BY