Staphylococcus aureus directly activates eosinophils via platelet‐activating factor receptor

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: atopic dermatitis;    colonization;    degranulation;    superoxide;    cytokines;    phagocytosis;   

DOI  :  10.1189/jlb.0112009

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ColonizationbySAisassociatedwithexacerbationofAD.EosinophilicinflammationisacardinalpathologicalfeatureofAD,butlittleisknownaboutpossibledirectinteractionbetweenSAandeosinophils.PAFRappearstobeinvolvedinphagocytosisofGram‐positivebacteriabyleukocytes.TheobjectiveofthisstudywastoinvestigatewhetherSAdirectlyinduceseosinophileffectorfunctionsviaPAFRinthecontextofADpathogenesis.Peripheralbloodeosinophilswereculturedwithheat‐killedSA,andEDNrelease,superoxidegeneration,andadhesiontofibronectin‐coatedplatesweremeasured.Cytokines,releasedinthesupernatants,werequantifiedbymultiplexbeadimmunoassays.FISH‐labeledSAwasincubatedwitheosinophilsandvisualizedbyconfocallaser‐scanningmicroscopy.PAFR‐blockingpeptideandPAFRantagonistsweretestedforinhibitoryeffectsonSA‐inducedreactions.SAinducedEDNreleaseandsuperoxidegenerationbyeosinophilsinadose‐dependentmanner.IL‐5significantlyenhancedSA‐inducedEDNrelease.IL‐5andIL‐17AsignificantlyenhancedSA‐inducedsuperoxidegeneration.SAenhancedeosinophiladhesiontofibronectin,whichwasblockedbyanti‐CD49d,andinducedeosinophilsecretionofvariouscytokines/chemokines(IL‐2R,IL‐9,TNFR,IL‐1β,IL‐17A,IP‐10,TNF‐α,PDGF‐bb,VEGF,andFGF‐basic).AfterincubationofeosinophilswithSA,FISH‐labeledSAwasvisualizedintheeosinophilsˈcytoplasm,indicatingphagocytosis.APAFR‐blockingpeptideandtwoPAFRantagonistscompletelyinhibitedthosereactions.Inconclusion,SAdirectlyinducedeosinophilactivationviaPAFR.BlockadeofPAFRmaybeanovel,therapeuticapproachforADcolonizedbySA...

【 授权许可】

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