International Journal of Clinical and Experimental Pathology | |
E-cadherin maintains the activity of neural stem cells and inhibits the migration | |
Dong Chen1  | |
关键词: Cadherins; E-cadherin; N-cadherin; neural stem cell; cell activity; cell migration; | |
DOI : | |
学科分类:生理学与病理学 | |
来源: e-Century Publishing Corporation | |
【 摘 要 】
Cadherins are important adhesion molecules that mediate adhesions and communications between cells. These molecules participate in the formation and maintenance of multicellular organisms including the stem cells. E-cadherin is one of the classic cadherins which is reported to be essential for the survival and self-renewal of embryonic stem cells. Moreover, it could induce cell proliferation inhibitory signaling to regulate cell proliferation. In our study, we over-expressed and silenced E-cadherin in NSCs by lentiviral ways. Transgenic cells were confirmed by both quantitative RT-PCR and western blot. Results of MTT assay showed that over-expression of E-cadherin could enhance the cell activity. Furthermore, we performed Transwell chamber assay to analyze its role in regulation of cell migration. The results showed that the migration percent of over-expression cells was lower than control. Our results indicated that E-caherin would maintain the stemness of NSCs and reduce cells migration.
【 授权许可】
CC BY-NC
【 预 览 】
Files | Size | Format | View |
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RO201901233744856ZK.pdf | 364KB | download |