Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment

American Journal of Translational Research

Jianqing Wang¹ 

关键词: Diabetic nephropathy;    protein kinase C;    telmisartan;    transforming growth factor-beta1;    vascular endothelial growth factor;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Aim: This study aims to investigate the localization and expression of protein kinase C-beta I and beta II in kidney cortex of diabetic nephropathy mice and their roles in telmisartan treatment. Methods: 18 mice were randomly divided into three groups: normal group, diabetic nephropathy group and telmisartan-treated group. The localization and expression of protein kinase C-beta I and beta II were measured with confocal immunofluorescence laser scanning microscopy, immunohistochemistry and western blotting. The expression of transforming growth factor-beta 1 and vascular endothelial growth factor in glomeruli was detected by immunohistochemistry. Results: Compared to the normal mice, the expression and localization of protein kinase C-beta I and beta II are differed in diabetic nephropathy mice, with increased expression of protein kinase C-beta I but decreased level of protein kinase C-beta II. Meanwhile, the expression of transforming growth factor-beta 1 and vascular endothelial growth factor showed increase in the glomeruli of diabetic nephropathy, compared to the controls. Also, protein kinase C-beta I exhibited a positive correlation to transforming growth factor-beta 1 (r = 0.649, P = 0.030), but no correlation to vascular endothelial growth factor (r = 0.387, P = 0.079). Telmisartan treatment exercised significant beneficial role in diabetic nephropathy, which is associated with protein kinase C-beta I, but not beta II. Conclusions: The expression and localization of protein kinase C-beta I and beta II differ in the diabetic nephropathy, and such difference is associated with the pathogeneses of diabetic nephropathy.

【 授权许可】

CC BY-NC