Effects of Allium victorialis leaf extracts and its single compounds on aldose reductase, advanced glycation end products and TGF-β1 expression in mesangial cells

BMC Complementary and Alternative Medicine

Jin Sook Kim²  Myung-Hwa Kim¹  Sea-Kwang Ku³  Song Yi Yu²  So-Jin Choi²  Ik Soo Lee²  Dong Ho Jung²  Young Sook Kim² 

[1] Present address: Korea Drug Development Fund, 21-1 Migeun-dong, Seodaemun-gu, Seoul 120-020, Republic of Korea;Korean Medicine-Based Herbal Drug Development Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon, Republic of Korea;Developmnent Team for the New Drug of Oriental Medicine, Daegu Haany University, Gyeongsan, Republic of Korea

关键词: Mouse mesangial cells;    transforming growth factor-beta1;    Diabetic nephropathy;    Advanced glycation end products;    Aldose reductase;    Allium victorialis;   

Others  :  1220939
DOI  :  10.1186/1472-6882-13-251

 received in 2013-07-02, accepted in 2013-09-26,  发布年份 2013

【 摘 要 】

Background

Accumulating evidences suggest that aldose reductase (AR) inhibitors and advanced glycation end product (AGE) formation inhibitors may prevent chronic hyperglycemia-induced long-term complication in diabetes. Transforming growth factor-beta1 (TGF-β1) plays an important role in the development of diabetic nephropathy. Allium species have been utilized in folk medicine throughout the world for the treatment of various physical disorders. However, the benefits of Allium victorialis (A. victorialis) against diabetic complications, especially nephropathy, have yet to be explored. In the present study, we investigated the protective effect of the compounds isolated from A. victorialis leaf on diabetic nephropathy.

Methods

In vitro AR activity, AGEs formation, and AGE-receptor for AGEs (RAGE) binding in human RAGE (hRAGE)-overexpressing cells were tested. High glucose-induced transforming growth factor-beta1 (TGF-β1) expression was also examined in mouse kidney mesangial cells (MMCs) cultured under high glucose.

Results

Of the isolated eight compounds from A. victorialis leaf extracts tested, quercitrin exhibited the most pronounced inhibitory effects on AR activity (IC₅₀ value of 0.17 μM) and AGEs formation (IC₅₀ value of 4.20 μM). Furthermore, quercitrin disrupted AGE-RAGE binding in a concentration-dependent manner in hRAGE-overexpressing cells. Additionally, of the eight compounds tested, ferulic acid significantly reduced high glucose-induced TGF-β1 expression and secretion in MMCs.

Conclusions

Our results suggest that active compounds isolated from A. victorialis leaf exhibit inhibitory effects on AR activity in rat lenses and AGE formation. Further, ferulic acid reduces TGF-β1 mRNA expression and secretion in MMCs under diabetic conditions. Thus, A. victorialis is a good candidate for the development of treatments for diabetic nephropathy.

【 授权许可】

   
2013 Kim et al.; licensee BioMed Central Ltd.

【 图 表 】

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