Emerging single‐cell technologies in immunology

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: sepsis;    infectious disease;    vaccines;   

DOI  :  10.1189/jlb.6RU0115-020R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Duringevolution,theimmunesystemhasdiversifiedtoprotectthehostfromtheextremelywidearrayofpossiblepathogens.Untilrecently,immuneresponsesweredissectedbyuseofglobalapproachesandbulktools,averagingresponsesacrosssamplesandpotentiallymissingparticularcontributionsofindividualcells.Thisisastronglylimitingfactor,consideringthatinitialimmuneresponsesarelikelytobetriggeredbyarestrictednumberofcellsatthevanguardofhostdefenses.Thedevelopmentofnovel,single‐celltechnologiesisamajorinnovationofferinggreatpromiseforbasicandtranslationalimmunologywiththepotentialtoovercomesomeofthelimitationsoftraditionalresearchtools,suchaspolychromaticflowcytometryormicroscopy‐basedmethods.Atthetranscriptionallevel,muchprogresshasbeenmadeinthefieldsofmicrofluidicsandsingle‐cellRNAsequencing.Attheproteinlevel,masscytometryalreadyallowstheanalysisoftwiceasmanyparametersasflowcytometry.Inthisreview,weexplorethebasisandoutcomeofimmune‐celldiversity,howgeneticallyidenticalcellsbecomefunctionallydifferent,andtheconsequencesfortheexplorationofhost‐immunedefenseresponses.Wewillhighlighttheadvantages,trade‐offs,andpotentialpitfallsofemerging,single‐cell‐basedtechnologiesandhowtheyprovideunprecedenteddetailofimmuneresponses...

【 授权许可】

CC BY