The anti‐inflammatory role of granulocyte colony‐stimulating factor in macrophage–dendritic cell crosstalk after Lactobacillus rhamnosus GR‐1 exposure

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: probiotics;    cytokines;    growth factors;    immunomodulation;   

DOI  :  10.1189/jlb.0810445

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

MΦsareimportantsensorycellsoftheinnateimmunesystemandregulateimmuneresponsesthroughreleasingdifferentcombinationsofcytokines.Inthisstudy,weexaminedwhethercytokinesreleasedbyMΦsinresponsetotheprobioticbacterialstrainGR‐1modulatetheresponsesofDCs.ThecytokineprofilereleasedbyGR‐1‐treatedMΦswascharacterizedbylowlevelsofTNF‐α,GM‐CSF,IL‐6,andIL‐12butveryhighlevelsofG‐CSF.GR‐1CMdidnotinduceexpressionofthesharedp40subunitofIL‐12andIL‐23andcostimulatorymoleculesCD80orCD86orincreaseTcellstimulatorycapacityinDCs.However,inG‐CSFR‐deficientDCsorafterantibody‐mediatedneutralizationofG‐CSF,GR‐1CMinducedIL‐12/23p40productionsignificantly,indicatingthatG‐CSFwithintheGR‐1CMinhibitsIL‐12/23p40productioninducedbyotherCMcomponents.GR‐1CMandrG‐CSFalsoinhibitedLPS‐inducedIL‐12productionatthemRNAandproteinlevels.TheinhibitionofIL‐12productionbyG‐CSFwasatleastinpartmediatedthroughinhibitionofJNKactivation.Finally,splenicDCsofGR‐1‐injectedmiceproducedlessIL‐12/23p40thanthoseofPBS‐injectedmiceinresponsetoLPSexvivo,andthiswasatleastpartiallydependentonexposuretoGR‐1‐inducedG‐CSFinvivo.Altogether,theseresultssuggestthatG‐CSFmodulatestheIL‐12/23p40responseofDCsinthecontextoftheprobioticGR‐1throughMΦ–DCcrosstalk...

【 授权许可】

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