| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Interaction of members of the heat shock protein‐70 family with the macrophage mannose receptor | |
| 关键词: receptor; binding proteins; cytoplasmic tail; | |
| DOI : 10.1189/jlb.1111562 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
ThemacrophageMRhasbeenthesubjectofinvestigationforover20years,andseveralimportantphysiologicalfunctionshavebeendescribed.However,themolecularmechanismsthatregulateMRsignalingandtraffickingduringtheseprocessesstillremainelusive.ThefocusofthecurrentpaperwastoidentifypotentialcellularMR‐interactingproteins.AninitialscreenofbindingproteinsinMR‐expressingcellswasperformedusingcoimmunoprecipitation,followedbyidentificationofmatchingpeptidesequencesusingproteomicsandMS.Themajorclassofbindingproteinsidentifiedbelongedtotheheatshockfamilyofproteins.ThespecificinteractionoftheMRwithHSP70familymemberswasvalidatedbyWesternblotanalysis,ligandbindingassays,andintracellularcolocalizationusingconfocalmicroscopy.AdditionalstudiesindicatedthatinhibitionoftheHSPBiPbytreatmentofcellswithEGCGreducedBiPinteractionwithandsurfaceexpressionoftheMR.StudiesofpossiblemotifswithinthecytoplasmictailofthereceptorsuggestedthatajuxtamembranedibasicsequencemaycontributetotheinteractionwithBiP.ThesefindingssuggestthatthemolecularassociationoftheMRwithHSP70familymembersviathereceptorcytoplasmictailmaycontributetoMRtraffickinginmacrophages...
【 授权许可】
CC BY
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201901230437569ZK.pdf | 1338KB |  download |
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