S100B Serves as a Ca2+ Sensor for ROS-GC1 Guanylate Cyclase in Cones but not in Rods of the Murine Retina

Cellular Physiology and Biochemistry

关键词: Synapse;    Cyclic GMP;    Calcium;    Retina;    Photoreceptors;    Knockout mice;    Guanylate cyclase;    S100B;    Phototransduction;   

DOI  :  10.1159/000338496

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Rod outer segment membrane guanylate cyclase (ROS-GC1) is a bimodal Ca²⁺ signal transduction switch. Lowering [Ca²⁺]_i from 200 to 20 nM progressively turns it “ON” as does raising [Ca²⁺]_i from 500 to 5000 nM. The mode operating at lower [Ca²⁺]_i plays a vital role in phototransduction in both rods and cones. The physiological function of the mode operating at elevated [Ca²⁺]_i is not known. Through comprehensive studies on mice involving gene deletions, biochemistry, immunohistochemistry, electroretinograms and single cell recordings, the present study demonstrates that the Ca²⁺-sensor S100B coexists with and is physiologically linked to ROS-GC1 in cones but not in rods. It up-regulates ROS-GC1 activity with a K_1/2 for Ca²⁺ greater than 500 nM and modulates the transmission of neural signals to cone ON-bipolar cells. Furthermore, a possibility is raised that under pathological conditions where [Ca²⁺]_i levels rise to and perhaps even enter the micromolar range, the S100B signaling switch will be turned “ON” causing an explosive production of CNG channel opening and further rise in [Ca²⁺]_i in cone outer segments. The findings define a new cone-specific Ca²⁺-dependent feature of photoreceptors and expand our understanding of the operational principles of phototransduction machinery.

【 授权许可】

CC BY-NC-ND